伯樂Bio-Rad1725301iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl
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伯樂Bio-Rad1725301iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl,產(chǎn)品簡(jiǎn)介:
產(chǎn)品品牌:伯樂Bio-Rad
產(chǎn)品名稱:iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl
產(chǎn)品貨號(hào):1725301
產(chǎn)品規(guī)格:50 µl
伯樂Bio-Rad1725301iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl,產(chǎn)品說明:
100 U, 2 U/µl, stand-alone enzyme, includes 5x reaction buffers, MgCl2 solution, DMSO
100件,2件/µl、 獨(dú)立酶,包括5倍反應(yīng)緩沖液,lv化鎂溶液,二甲基亞砜
IPROP高保真度DNA聚合酶是一種*的熱球菌樣校對(duì)酶,融合到Sso7d dsDNA結(jié)合蛋白中,以創(chuàng)建一種熱穩(wěn)定性融合聚合酶,精確地放大來自多種DNA模板的長(zhǎng)產(chǎn)品。
IPROVE高保真度DNA聚合酶的特點(diǎn)和優(yōu)點(diǎn):
高保真度-IPRO頂DNA聚合酶比Taq聚合酶準(zhǔn)確52倍
速度-高處理能力顯著減少擴(kuò)展(15–30秒/kb)和總反應(yīng)時(shí)間
成功擴(kuò)增出高產(chǎn)的長(zhǎng)產(chǎn)物-在更短的時(shí)間內(nèi)擴(kuò)增出高達(dá)37KB的片段,酶的含量也較少(0.25–1單位/反應(yīng))
IPROVE聚合酶可在易于使用的主混合和一個(gè)PCR試劑盒中提供,并配有對(duì)照。
高保真和普通PCR試劑
iProofTMHigh- Fidelity DNA Polymerase
高保真一比普通Taq酶錯(cuò)配率低52倍
*保真酶融合dsDNA結(jié)含蛋白Sso7d
高速度一合成速度達(dá)15~30秒/kb
長(zhǎng)距離PCR能力強(qiáng)-只需很少的酶量(0.25~1U)即可擴(kuò)增37 kb的產(chǎn)物
還可提供帶高GC模板緩沖液的2x預(yù)混液
ROX惰性對(duì)照染料
對(duì)于ABI PRISM7000 7300、 7700和7900,, 適用50倍稀釋液
對(duì)于580~585 nm激發(fā)波長(zhǎng)的光學(xué)系統(tǒng),比如Mx3000P、4000、 3005 或ABI 7500,則應(yīng)用750倍稀釋液。
Bio-Rad系列實(shí)時(shí)PCR儀,一般不需要內(nèi)對(duì)照
dNTP Mix
性能穩(wěn)定,適合高效率PCR和real-time PCR反應(yīng)
耐受多次反復(fù)凍融和熱循環(huán)
iTaqTMDNA聚合酶
抗體熱啟動(dòng)DNA聚合酶,95°C3 分鐘快速激活
用于普通PCR和real-timePCR反應(yīng),減少非特異擴(kuò)增和引物二聚體
iProof High-Fidelity DNA Polymerase | Number of Reactions | Catalog Number |
iProof High-Fidelity PCR Kit | 50 U (2 U/µl) | 1725330 |
iProof High-Fidelity PCR Kit | 200 U (2 U/µl) | 1725331 |
iProof HF Master Mix | 100 x 50 µl reactions | 1725310 |
iProof GC Master Mix | 100 x 50 µl reactions | 1725320 |
iProof High-Fidelity DNA Polymerase | 20 U (2 U/µl) | 1725300 |
iProof High-Fidelity DNA Polymerase | 100 U (2 U/µl) | 1725301 |
iProof High-Fidelity DNA Polymerase | 500 U (2 U/µl) | 1725302 |
伯樂Bio-Rad1725301iProof™ High-Fidelity DNA Polymerase, 100 U (2 U/µl), 50 µl,產(chǎn)品訂購(gòu)信息:
iProof High Fidelity DNA Polymerase, Master Mixes, and Buffers
伯樂Bio-Rad 1725300 iProof High-Fidelity DNA Polymerase, 2 U/ul, 20 U, includes 5x reaction buffers, MgCl solution, DMSO
伯樂Bio-Rad 1725301 iProof High-Fidelity DNA Polymerase, 2 U/ul, 100U
伯樂Bio-Rad 1725302 iProof High- Fidelity DNA Polymerase, 2U/ul, 500U
伯樂Bio-Rad 1725330 iProof High-Fidelity PCR Kit, 2 U/ul, 50 U, includes 5x reaction buffers, MgCl solution, DMSO
伯樂Bio-Rad 1725310 iProof HF Master Mix, 2.5 ml, 100 X 50 ul reactions (for highest fidelity with most templates)
伯樂Bio-Rad 1725320 iProof GC Master Mix, 2.5 ml, 100 x 50 ul reactions (for GC-rich templates)
伯樂Bio-Rad 1725391 5x iProof HF Buffer, 1.5 ml (for highest fdelity with most templates)
伯樂Bio-Rad 1725392 5x iProof GC Buffer, 1.5 ml (for GC-rich templates)
伯樂Bio-Rad 1725393 5x iProof HPLC HF Buffer, 1.5 ml
伯樂Bio-Rad 1725394 5x iProof HPLC GC Buffer, 1.5 ml
ROX Passive Reference Dye
伯樂Bio-Rad 1725858 ROX Passive Reference Dye, 0.5ml
dNTP Mix
伯樂Bio-Rad 1708874 dNTP Mx 20uprenied sluton, cotains 10mM each dNTP (dATP. dCTP.dGTP.TTP
iTaq DNA Polymerase
伯樂Bio-Rad 1708870 iTaq DNA Polymerase, 5Uu, icues 250Upoymeree. 1.25 ml 10x PORuffe
伯樂Bio-Rad 1708875 iTaq DNA Polymerase, 5U/ul includes 5,000Upolymerase, 25 ml 10xPCRbuffer
MgCl2
伯樂Bio-Rad 1708872 MgCl, soution 50mM, 1.25ml