GK021 ScienCell 人類T細(xì)胞受體信號通路qPCR檢測試劑盒,Human T Cell Receptor Signaling Pathway qPCR Array Kit
ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。
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GK021 ScienCell人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit，產(chǎn)品簡介：
產(chǎn)品品牌; ScienCell
產(chǎn)品貨號：GK021
產(chǎn)品名稱：GK021 ScienCell人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit
產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well
GK021 ScienCell人類T細(xì)胞受體信號通路qPCR檢測試劑盒，Human T Cell Receptor Signaling Pathway qPCR Array Kit， 產(chǎn)品說明：
ScienCell 的 GeneQuery™ 人 T 細(xì)胞受體信號通路 qPCR 陣列試劑盒 （GQH-TRS） 旨在促進(jìn)參與 T 細(xì)胞受體信號通路的 88 個關(guān)鍵基因的基因表達(dá)譜分析。如何根據(jù)其功能對所包含的基因進(jìn)行分組的簡要示例如下所示：
T細(xì)胞受體復(fù)合物成分：CD3D，CD3E，CD3G
輔助受體：CD4、CD8A、CD8B
輔助抑制劑：PDCD1、CTLA4
共刺激物：ICOS，CD28
激酶：FYN，ZAP70
MAPK途徑：KRAS，HRAS，NRAS，RAF1，MAP2K1，MAPK1，MAPK3
PI3K/AKT 通路：PI3K、AKT1、AKT2
IKK/NFKB途徑：NFKB，DAG1，BCL10，CARD11，MAP3K7，MALT1，CHUK，IKBKB，IKBKG
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可以特異性識別并有效擴增靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+，不含DMSO）;（ii）除非另有說明，否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。
ScienCell's GeneQuery™ Human T Cell Receptor Signaling Pathway qPCR Array Kit (GQH-TRS) is designed to facilitate gene expression profiling of 88 key genes involved in T cell receptor signaling pathway. Brief examples of how included genes may be grouped according to their functions are shown below:
· T cell receptor complex components: CD3D, CD3E, CD3G
· Co-receptors: CD4, CD8A, CD8B
· Co-inhibitors: PDCD1, CTLA4
· Co-stimulators: ICOS, CD28
· Kinases: FYN, ZAP70
· MAPK pathway: KRAS, HRAS, NRAS, RAF1, MAP2K1, MAPK1, MAPK3
· PI3K/AKT pathway: PI3Ks, AKT1, AKT2
· IKK/NFKB pathway: NFKBs, DAG1, BCL10, CARD11, MAP3K7, MALT1, CHUK, IKBKB, IKBKG
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.
基因查詢™人 T 細(xì)胞受體信號通路 qPCR 陣列板布局*（8 個粗體和斜體控件）
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | AKT1 | CD274 | CD8A | DYRK1A | HRAS | MALT1 | NCK1 | NFKBIE | PDPK1 | PPP3CB | RELA | β-actin |
B | AKT2 | CD28 | CD8B | DYRK2 | ICOS | MAP2K1 | NCK2 | NRAS | PIK3CA | PPP3R1 | SOS1 | GAPDH |
C | BCL10 | CD3D | CDC42 | FOS | IKBKB | MAP2K7 | NFATC1 | NRON | PIK3CB | PRKCA | SOS2 | LDHA |
D | CALM1 | CD3E | CDK4 | FYN | IKBKG | MAP3K7 | NFATC2 | PAK1 | PIK3R1 | PRKCQ | TEC | NONO |
E | CARD11 | CD3G | CHUK | GRAP2 | ITK | MAP3K8 | NFATC3 | PAK2 | PIK3R2 | PTCRA | TNF | PPIH |
F | CBL | CD4 | CTLA4 | GRB2 | JUN | MAPK1 | NFKB1 | PAK3 | PIK3R3 | PTPN6 | VAV1 | GDC |
G | CBLB | CD80 | DAG1 | GSK3A | KRAS | MAPK3 | NFKBIA | PAK4 | PLCG1 | RAF1 | VAV2 | PPC |
H | CBLC | CD86 | DLG1 | GSK3B | LCP2 | MAPK9 | NFKBIB | PDCD1 | PPP3CA | RASGRP1 | ZAP70 | NTC |
提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新
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