GK084 ScienCell 人體軸突導(dǎo)向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit
ScienCell 研究實驗室(ScienCell)是一家生物技術(shù)公司,其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞,細(xì)胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細(xì)胞來源的RNA,cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量,實現(xiàn)細(xì)胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥,公司成立于1999年。
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GK084 ScienCell人體軸突導(dǎo)向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit,產(chǎn)品簡介:
產(chǎn)品品牌: ScienCell
產(chǎn)品貨號:GK084
產(chǎn)品名稱:GK084 ScienCell人體軸突導(dǎo)向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit
產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well
GK084 ScienCell人體軸突導(dǎo)向qPCR檢測試劑盒,Human Axon Guidance qPCR Array kit, 產(chǎn)品說明:
ScienCell的GeneQuery™人類軸突引導(dǎo)qPCR陣列(GQH-AXN)分析了88個參與神經(jīng)尋路的關(guān)鍵基因。 在神經(jīng)發(fā)育過程中,神經(jīng)元發(fā)送投影(也稱為軸突)以到達(dá)目標(biāo)并傳輸電信號。 軸突遵循特定的路徑,潛在的尋路機(jī)制是一個不斷增長的研究領(lǐng)域。 以下是如何根據(jù)其信號通路或蛋白質(zhì)類別對所包含基因進(jìn)行分組的簡要示例:
腎上腺素: EFNA1, EFNA5, EFNB3, EPHA1, EPHB1
信號素: SEMA3A, SEMA4D, SEMA5A, SEMA6B, SEMA7A
WNT 信令: WNT4, WNT5A, SHH, SMO, FZD3
Netrin信令:NTN1,NTN4,NTNG1,NTNG2,DCC,UNC5A
細(xì)胞粘附分子:CAMK2A、CAMK2B、CAMK2D、CAMK2G
GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可識別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計的引物確保:(i)qPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+且不含DMSO);(ii)除非另有說明,否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;(iii)只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。
ScienCell's GeneQuery™ Human Axon Guidance qPCR Array (GQH-AXN) profiles 88 key genes involved in neural pathfinding. During neural development, neurons send projections otherwise known as axons to reach targets and transmit electric signals. Axons follow specific paths and the underlying pathfinding mechanism is an area of growing research. Below are brief examples of how included genes may be grouped according to their signaling pathways or protein classes:
· Ephrins: EFNA1, EFNA5, EFNB3, EPHA1, EPHB1
· Semaphorins: SEMA3A, SEMA4D, SEMA5A, SEMA6B, SEMA7A
· WNT signaling: WNT4, WNT5A, SHH, SMO, FZD3
· Netrin signaling: NTN1, NTN4, NTNG1, NTNG2, DCC, UNC5A
· Cell adhesion molecules: CAMK2A, CAMK2B, CAMK2D, CAMK2G
GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.
GeneQuery™ 人類軸突引導(dǎo) qPCR 陣列板布局*(8 個粗體和斜體對照)
1 | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | |
A | ABL1 | CAMK2B | EFNA1 | EPHA1 | EPHB3 | NRP1 | RAC1 | SEMA3D | SEMA4F | SLIT2 | TRPC6 | β-actin |
B | ABLIM3 | CAMK2D | EFNA2 | EPHA2 | EPHB4 | NRP2 | RAF1 | SEMA3E | SEMA4G | SLIT3 | UNC5A | GAPDH |
C | ARHGEF12 | CAMK2G | EFNA3 | EPHA3 | F2RL2 | NTN1 | ROBO1 | SEMA3F | SEMA5A | SMO | UNC5B | LDHA |
D | BMP7 | CFL1 | EFNA4 | EPHA4 | FES | NTN4 | ROBO2 | SEMA3G | SEMA6B | TRPC1 | UNC5C | NONO |
E | BMPR1B | CFL2 | EFNA5 | EPHA5 | FYN | NTNG1 | ROCK1 | SEMA4A | SEMA6D | TRPC1 | UNC5D | PPIH |
F | BMPR2 | CXCR4 | EFNB1 | EPHA7 | FZD3 | NTNG2 | RYK | SEMA4B | SEMA7A | TRPC3 | WNT4 | GDC |
G | BOC | DCC | EFNB2 | EPHB1 | NCK1 | PAK2 | SEMA3A | SEMA4C | SHH | TRPC4 | WNT5A | PPC |
H | CAMK2A | DPYSL2 | EFNB3 | EPHB2 | NFATC2 | PAK4 | SEMA3C | SEMA4D | SLIT1 | TRPC5 | WNT5B | NTC |
提示:基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新
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