GK089 ScienCell 人類Ephrin信號qPCR檢測試劑盒，Human Ephrin Signaling qPCR Array Kit

ScienCell 研究實驗室（ScienCell）是一家生物技術(shù)公司，其使命是實驗和治療用細(xì)胞和細(xì)胞相關(guān)產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細(xì)胞，細(xì)胞培養(yǎng)基和試劑，培養(yǎng)基添加因子，細(xì)胞來源的RNA，cDNA和蛋白質(zhì)。ScienCell的科學(xué)家們以改善生活質(zhì)量，實現(xiàn)細(xì)胞的能力，打擊人類退化性疾病為目的研究和開發(fā)各種細(xì)胞治療策略。ScienCell總部設(shè)在美國加利福尼亞州圣地亞哥，公司成立于1999年。

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GK089 ScienCell人類Ephrin信號qPCR檢測試劑盒，Human Ephrin Signaling qPCR Array Kit，產(chǎn)品簡介：

產(chǎn)品品牌： ScienCell

產(chǎn)品貨號：GK089

產(chǎn)品名稱：GK089 ScienCell人類Ephrin信號qPCR檢測試劑盒，Human Ephrin Signaling qPCR Array Kit

產(chǎn)品規(guī)格：One 96-well plate, one 20ul reaction per well

GK089 ScienCell人類Ephrin信號qPCR檢測試劑盒，Human Ephrin Signaling qPCR Array Kit， 產(chǎn)品說明：

ScienCell的GeneQuery™人類ephrin信號qPCR芯片試劑盒（GQH-EPH）分析了88個參與ephrin信號傳導(dǎo)和信號轉(zhuǎn)導(dǎo)的關(guān)鍵基因。 Ephrin 信號傳導(dǎo)參與軸突引導(dǎo)、血管生成、細(xì)胞遷移、胚胎發(fā)育和分割等發(fā)育過程。 腎上腺素配體和受體根據(jù)其結(jié)構(gòu)和結(jié)合親和力細(xì)分為兩類：mahuangsu-A和mahuangsu-B。 ephrin 信號的一個更dute的方面是它可以雙向表達(dá)為“正向信號"和“反向信號"。 如何根據(jù)其功能對所包含的基因進(jìn)行分組的簡要示例如下所示：

信號受體：EPHA2、EPHA4、EPHB1、EPHB2、EPHB4

配體：EFNA1、EFNA2、EFNA5、EFNB1、EFNB2

細(xì)胞骨架動力學(xué)：ACTB、ACTG1、ACTN1、CFL1、DNM1

囊泡販運：AP2B1、AP2S1、APH1A、CAV1、CLTA

止血： FN1， FYN， ITGAVM ITGB1， LYN

GeneQuery™ qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒，每個孔包含一個引物組，可識別并有效擴(kuò)增特定靶基因的 cDNA。精心設(shè)計的引物確保：（i）qPCR分析中的優(yōu)良退火溫度為65°C（含2 mM Mg2+且不含DMSO）;（ii）除非另有說明，否則引物集可識別靶基因的所有已知轉(zhuǎn)錄本變體;（iii）只有一個基因被擴(kuò)增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進(jìn)行了驗證。

ScienCell's GeneQuery™ Human Ephrin Signaling qPCR Array Kit (GQH-EPH) profiles 88 key genes involved in ephrin signaling and signal transduction.  Ephrin signaling is involved in such developmental processes as axonal guidance, angiogenesis, cell migration, embryonic development, and segmentation.  Ephrin ligands and receptors are subdivided into two classes based on their structure and binding affinities: ephrin-A and ephrin-B.  A more unique aspect of ephrin signaling is that it can occur bidirectionally expressing as both a “forward signal" and a “reverse signal."  Brief examples of how included genes may be grouped according to their function are shown below:

·         Signaling receptors: EPHA2, EPHA4, EPHB1, EPHB2, EPHB4

·         Ligands: EFNA1, EFNA2, EFNA5, EFNB1, EFNB2

·         Cytoskeletal dynamics: ACTB, ACTG1, ACTN1, CFL1, DNM1

·         Vesicle Trafficking: AP2B1, AP2S1, APH1A, CAV1, CLTA

·         Hemostasis: FN1, FYN, ITGAVM ITGB1, LYN

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that recognizes and efficiently amplifies a specific target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+and no DMSO); (ii) the primer set recognizes all known transcript variants of the target gene, unless otherwise noted; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis and gel electrophoresis.

GeneQuery™ 人 Ephrin 信號 qPCR 陣列板布局*（8 個粗體和斜體對照）

提示：基因選擇可能會根據(jù)新的研究和開發(fā)進(jìn)行更新

ScienCell基因表達(dá)分析產(chǎn)品訂購信息：

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