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ScienCellGK092人胰腺星狀細胞生物學qPCR檢測試劑盒
更新時間:2023-01-03   點擊次數(shù):567次

GK092 ScienCell 人胰腺星狀細胞生物學qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

ScienCell 研究實驗室(ScienCell)是一家生物技術公司,其使命是實驗和治療用細胞和細胞相關產(chǎn)品的研究和發(fā)展。 ScienCell為研究界提供各種高質(zhì)量的正常的人類和動物細胞,細胞培養(yǎng)基和試劑,培養(yǎng)基添加因子,細胞來源的RNAcDNA和蛋白質(zhì)。ScienCell的科學家們以改善生活質(zhì)量,實現(xiàn)細胞的能力,打擊人類退化性疾病為目的研究和開發(fā)各種細胞治療策略。ScienCell總部設在美國加利福尼亞州圣地亞哥,公司成立于1999年。

北京諾博萊德作為ScienCell品牌的經(jīng)銷商,為客戶提供,正規(guī)進口,貨期穩(wěn)定,折扣好的服務標準。

GK092 ScienCell人胰腺星狀細胞生物學qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit,產(chǎn)品簡介:

產(chǎn)品品牌: ScienCell

產(chǎn)品貨號:GK092

產(chǎn)品名稱:GK092 ScienCell人胰腺星狀細胞生物學qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit

產(chǎn)品規(guī)格:One 96-well plate, one 20ul reaction per well


GK092 ScienCell人胰腺星狀細胞生物學qPCR檢測試劑盒,Human Pancreatic Stellate Cell Biology qPCR Array Kit, 產(chǎn)品說明:

ScienCell GeneQuery™ 人類胰腺星狀細胞生物學 qPCR 陣列試劑盒 (GQH-PSB) 旨在促進參與 (i) 人胰腺星狀細胞活化的 88 個關鍵基因的基因表達譜;(二)視黃醇代謝;iii)細胞外基質(zhì)(ECM)合成和重塑;以及(iv)胰腺星狀細胞相關疾病。如何根據(jù)其功能對所包含的基因進行分組的簡要示例如下所示:  

胰腺星狀細胞標志物:ACTA2GFAP,COL11A1,DES,NGFR,POSTN,ITGA7,CTGF

胰腺星狀細胞活化:ICAM1MMP13,RELN,TIMP1,SYP,CDH1,CDH2,RARA,PDGF,TGFB1,MMP2

視黃醇代謝: RBP1 RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26s

ECM合成和重塑:TGFB1,COL4A2,層粘連蛋白亞基,SPARC,VCAN,CTGF

細胞因子生產(chǎn):IL1A,IL1B,IL6PDGF,TGFBs,TNF

p53 信號通路: CCNB1, CDK1, CCNE1 RRM2, GADD45G, CDK6, CHEK1, CDKN1A, EI24, ZMAT3

胰腺纖維化和癌癥發(fā)展:ACVR1BCCKBR,CDKN2A,CELSR3EGFR,GNL3,PALLD,RBBP8RIMS1,SMAD4,STK11

GeneQuery qPCR 芯片試劑盒采用 96 孔板格式的 qPCR 就緒,每個孔包含一個引物組,可以特異性識別并有效擴增靶基因的 cDNA。精心設計的引物確保:(iqPCR分析中的優(yōu)良退火溫度為65°C(含2 mM Mg2+,不含DMSO;ii)除非另有說明,否則引物組可識別靶基因的所有已知轉(zhuǎn)錄本變體;iii)只有一個基因被擴增。每個引物組都已通過qPCR和熔解曲線分析和凝膠電泳進行了驗證。

ScienCell's GeneQuery™ Human Pancreatic Stellate Cell Biology qPCR Array Kit (GQH-PSB) is designed to facilitate gene expression profiling of 88 key genes involved in (i) human pancreatic stellate cell activation; (ii) retinol metabolism; (iii) extracellular matrix (ECM) synthesis and remodeling; and (iv) pancreatic stellate cell related disorders. Brief examples of how included genes may be grouped according to their functions are shown below:    

·         Pancreatic stellate cell markers: ACTA2, GFAP, COL11A1, DES, NGFR, POSTN, ITGA7, CTGF

·         Pancreatic stellate cell activation: ICAM1, MMP13, RELN, TIMP1, SYP, CDH1, CDH2, RARA, PDGFs, TGFB1, MMP2

·         Retinol metabolism: RBP1, RBP2, RBP4, RBP5, CRABP1, CRABP2, LRAT, CYP26s

·         ECM synthesis and remodeling: TGFB1, COL4A2, Laminin subunits, SPARC, VCAN, CTGF

·         Cytokine production: IL1A, IL1B, IL6, PDGFs, TGFBs, TNF

·         p53 signaling pathway: CCNB1, CDK1, CCNE1, RRM2, GADD45G, CDK6, CHEK1, CDKN1A, EI24, ZMAT3

·         Pancreatic fibrosis and cancer development: ACVR1B, CCKBR, CDKN2A, CELSR3, EGFR, GNL3, PALLD, RBBP8, RIMS1, SMAD4, STK11

GeneQuery™ qPCR array kits are qPCR ready in a 96-well plate format, with each well containing one primer set that can specifically recognize and efficiently amplify a target gene's cDNA. The carefully designed primers ensure that: (i) the optimal annealing temperature in qPCR analysis is 65°C (with 2 mM Mg2+, and no DMSO); (ii) the primer set recognizes all known transcript variants of target gene, unless otherwise indicated; and (iii) only one gene is amplified. Each primer set has been validated by qPCR with melt curve analysis, and gel electrophoresis.

基因查詢™人胰腺星狀細胞生物學qPCR陣列板布局*8 個粗體和斜體控件)


1

2

3

4

5

6

7

8

9

10

11

12

A

ACTA2

CDK1

COL2A1

DSP

HIF1A

LAMA2

MMP13

PALLD

RBP1

SPARC

THBS2

β-actin

B

ACTG2

CDK6

COL4A2

EGFR

ICAM1

LAMA3

MMP2

PDGFA

RBP2

SPP1

TIMP1

GAPDH

C

ACVR1B

CDKN1A

CRABP1

EI24

IL1A

LAMB1

MYH11

PDGFB

RBP4

STK11

TNC

LDHA

D

CCKBR

CDKN2A

CRABP2

FOXF1

IL1B

LAMB3

MYH9

PDGFC

RBP5

SYP

TNF

NONO

E

CCNB1

CELSR3

CTGF

GADD45G

IL6

LAMC1

NES

PDGFD

RELN

TGFB1

VCAN

PPIH

F

CCNE1

CHEK1

CYP26A1

GFAP

ITGA7

LHX2

NFKB1

POSTN

RIMS1

TGFB2

VIM

GDC

G

CDH1

CLEC3B

CYP26B1

GNL3

KLF6

LOX

NFKB2

RARA

RRM2

TGFB3

VTN

PPC

H

CDH2

COL11A1

DES

HAS1

LAMA1

LRAT

NGFR

RBBP8

SMAD4

TGFBI

ZMAT3

NTC

提示:基因選擇可能會根據(jù)新的研究和開發(fā)進行更新

ScienCell基因表達分析產(chǎn)品訂購信息:

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